A Collagen Shot serving is 1 pouch (roughly 1 fluid ounce) that contains 10g of bovine hydrolyzed collagen with 35mg of vitamin C. There are 15 servings per box, and they are best taken 30-60 minutes before you train and away from caffeine. The next best place to take Collagen Shots is before you go to bed. We generally recommend to take supplements with a little food in your belly as taking supplements on an empty stomach may cause upset.